New and Unexplored Infection Laboratory

List of research projects carried out in the laboratory:

1. Name of research project: “The development of molecular-genetic test-systems for detection of tuberculosis agent and genotyping of mycobacterium”.
Project start and accomplishment data: 2001.01-2003.12
Project financing: Academy of medical Science Ukraine.
Head of the project: Volyansky Y.L., Director IMI, Dr. Sc., Prof.; Limansky A.P., Principal Scientist of LNNVWID, Ph. D.

2. Name of research project: “The development a express diagnostic and treatment control method (polymerase chain reaction based) for legionella infection”.
Project start and accomplishment data: 2002.01-2004.12
Project financing: Academy of medical Science Ukraine.
The collaborating organization: Ltd “Bioanalytical technolohies” (Kharkov, UA),
E-mail: bat@kharkov.com
Head of the project: Pokhil S.I., Director Scientific IMI, Head of LNNVSID, Ph. D., Principal Scientist; Shagun S.V., Director Ltd “B.A.T.”.

3. Name of research project: “Stady of Bacillus antracis genome organization and desing of molecular test-systems for detection and typing”.
Project start and accomplishment data: 2002.01-2004.12
Project financing: Academy of medical Science Ukraine.
Head of the project: Volyansky Y.L., Director IMI, Dr. Sc., Prof.; Limansky A.P., Principal Scientist of LNNVWID, Ph. D.

4. Name of research project: “Effect of laser radiation on biological properties (expression of the pathogenic factors) of the agents of infectious diseases”.
Project start and accomplishment data: 2001.01-2003.12
Project financing: Joint-Stock Company “Center of Laser and Microwave Technologies” (Kharkov, UA).
The collaborating organization:Research Institute for Laser Biology and Laser Medicine, Kharkov State University (RILBLM).
E-mail: Laser@VL Kharkov.ua; URL: http://www.medlaser.com.ua.
Head of the project: Pokhil S.I., Director Scientific IMI, Head of LNNVSID, Ph. D., Principal Scientist; Korobov A.M., Director RILBLM, Ph. D.

Sergey I. Pokhil
Head of Department

Stuff:

• Sergey I. Pokhil, Head of Department,
  Senior Research Scientist, Ph.D. of medicine
  Work address: Mechnicov Institute of Microbiology and Immunology, 14, Pushkinskaya Str., Kharkov, 61057, Ukraine
  Home address: App.99, Frunse Pr., 26, Kharkov, 61007, Ukraine
  E-mail: imiamn@mail.ru
• Alexandr P. Limansky, Research scientist, Ph.D. of biology.
  Sc. interests: molecular biology, molecular diagnostics, polymerase chain reaction, atomic forse microscopy
• Elena N. Timchenko, research scientist
  Sc.interests: molecular biology, DNA restriction patterns, epidemiological typing bacteria.
• Natalia P. Volyanskaya, research scientist - clinical microbiology, methods of determination of antibacterial preparations effect on microorganisms
  Sc.interests: clinical microbiology, methods of determination of antibacterial preparations effect on microorganisms.
• Igor P. Udin, íàó÷.ñîòð.
  Sc.interests: clinical microbiology, method of determination of microorganisms viabilities, methods of determination of desinfectants effect.
• Tatyana A. Mishukova, assistent

Main publications:

1. Pokhil S.,Smelanskaya M. Method of sample preparation for electron microscopy study of fibriae structures of bacteria. //Klinikal and laboratory diagnostics.1993;(5);22-24
2. Tsiganenko A.,Pokhil S.Methods of bacteria classifications: modern state of the problem.// The medicine problem bulletin.1995;(9);15-24
3. Tsiganenco A.,Pokhil S. Thoughts on modern classification and nomenclature for bacteria of Salmonella species.//The medicine problem bulletin.1995;(10);89-100
4. Krasovsky V.,Volansky Yu.,Pokhil S. Characteristics of biological properties of Listeria strains isolates in Ukraine and Belgium./Microbiol.J.1996;58(3),10-17
5. Pokhil S.Species of enterobacteria new to medicine.//Microbiol.J.1996;58(3);94-103.
6. Pokhil S.,Korniyenko I.,Pokhil Sv. Biological properties and medicinal significance of Rahnella aquatilis. //Bulletin of medicine and biology problems.1996;(4);129-140
7. Pokhil S. Rarely occuring, atypical and new enteerobacteria. //Microbiol.J.1996;58(4);93-104
8. Tsiganenko A.,Pokhil S. To 100-th anniversary of the discovery of Widal's reactions of agglutination Chistory of serodiagnostics development.//Microbiol.J.1996;58(5);107-109
9. Pokhil S.The biological properties of adsorbed and nonadsorbed OH-agglutinating sera for the identification of Rahnella aquatilis bacteria.//Microbiol.J.1997;59(1);70-76.
10. Pokhil S.,Mackiy N. Criteria of etiological diagnostics of the acute intestinal infections caused by Rahnella aquatilis// Infecious Diseas.1997;(3).10-13
11. Pokhil S. Bacteriological diagnostics of diseases caused by Rahnella aquatilis.//Clin.Lab.Diag.1997.(8).44-47.
12. Timchenko Ye.,Volansky Yu.,Pokhil S.Analysis of restriction patterns of DNA as a method of modern epidemiological typing of the pathogenic agents of infection diseases.//Bulletin of medicine and biology problems.1997;(32);78-86
13. Pokhil S.,Savin R. Application of divice "Pulse-Gep1"for conducting gel-electrophoresis in the pulsatins field.// Ukrainian Journal of Medical Equipment and Technology.1997;(3-4);71-75.
14. Pokhil S.,Pokhil Sv.Intestinal CLV-infection in the Ukraine.// Infections Diseases.1998;(1);10-14
15. Tsiganenko A.,Timchenko Ye.,Pokhil S.DNA restriction patterns as a modern method of bacteria classification.//Bulletin of medicine and biology problems.1998;(2);16-26
16. Pokhil S.,Krasovsky V.,Timchenko Ye. Assessing the weight of inoculate applied by Semenikhina's replicator.// Clin.Lab.Diag.1998;(7);34-36
17. Pokhil S.Biological properties of Rachnella aquatilis strains isolated in different regions.// Microbiol.J.1998;60(3);31-36.
18. Pokhil S.,Derkach S.,Pivnenco T.,Pertskhalava T. Identification of species of Enterobacteriace family producing hydrogen sulphoxide.//Microbiol.J.1998;60(4);3-14
19. Pokhil S.I. Properties of Pragia fontium bacteria isolated in the territory of Ukraine and Czechia. Microb.Z. (Ukraine). 2000, 62(2); 3-10.
20. Krasovsky V.V., Vasilyev N.V., Derkach S.A., Pokhil S.I. Results of the five-year study of listeriosis in the Ukraine. Zh. Microbiol. (Moscow). 2000; (3); 80-85.
21. Krasovsky V.V., Nadtoka V.L., Pokhil S.I. Listeria carriership: a sanitation method. Zh. Microbiol. (Moscow). 2000; (3); 18-20.
22. Limansky A.P., Limanskaya O.Yu., Belozersky V.I., Timchenko Ye.N., Pokhil S.I. Development and trials of polymerase chain reaction for detection of meningococcal infection agent. Clin. Lab. Diag. (Moscow). 2000; (4); 44-47.
23. Krasovsky V.V., Pokhil S.I., Limansky A.P., Limanskaya O.Yu., Timchenko Ye.N. Development and trials of polymerase chain reaction for detection of Listeria. Clin. Lab. Diag. (Moscow). 2000; (6); 37-41.
24. Pokhil S.I. Evolutionary Phylogenetic Classification of Infections Diseases. - Kharkov, 2000. - 27 p.
25. Pokhil S.I. Microbiological characteristics of Rahnella aquatilis srtains isolated from patients with diarrea. Vesnic Sum. State Univ. 2001. 32 (11); 132-137.
26. Timchenko Ye.N. Pheno- and genotypic of Staphylococcus aureus. Vestnik Sum. State Univ. 2001; 32(11); 143-147.
27. Volyanskaya N.P., Grechko V.A. Some tendencies of chemotherapy and chemoprophylactics of acute inflammatory diseasces in modern clinics. Vestnik Sum. State Univ. 2001; 32(11); 106-113.
28. The method of isolation, identification and longtermin deposition of pure cultures of Rahnella aquatilis/ Pokhil S.I., Osolodchenko T.P., Volyanska N.P. et al. In : “Methods of recieving of pure microorganisms cultures and of their long-time saving in collections: Works of the stuff of the museum of microorganisms to be pathogenetic for human”. - Kiev: Znannya, 2000. - P. 66-80.
29. The method of isolation, identification and longtermin deposition of pure cultures of Listeria/ Krasovsky V.V., Pokhil S.I., Osolodchenko T.P. et al. In : “Methods of recieving of pure microorganisms cultures and of their long-time saving in collections: Works of the stuff of the museum of microorganisms to be pathogenetic for human”. - Kiev: Znannya, 2000. - P. 49-65.
30. Culture catalogue: Bacteria. Mycoplasma. Chlamydia. Yeasts-like fungi. Mycelious fungi. Cell Cultures/ Selnikova O.P., Mavrov I.I., Boschenko Yu.A., Pokhil S.I., - Kiev: Znannya, 2000. - 119 p.
31. Timchenko Ye.N. Genotypic of hospital strains of Staphylococcus aureus by analysis of the length polymorphism of restrictions of chromosome DNA/ Vestnic Khar. State. Univ. 2002
32. Limanska O. Yu., Limansky A. P., Bezugly N. D. Preimplantation embryo sexing of farm animals by polymerase chain reaction. Biotechnology. (in Russian). 2000; (2); 66-72.
33. Limansky A.P. Visualization of cruciform structure in supercoiled DNA by atomic force microscopy. – Biofizika. (in Russian). 2000; 45(6); 1039-1043.
34. Limansky A.P. Investigation of aminomodified mica as a substrate for nucleic acids atomic force microscopy. Biopolymers and Cell.(in Russian). 2001; 17(4); 292 – 297.
35. Limanska O.Yu., Limansky A.P. Test – system for cattle embryos gene typing. – Biological Bulletin. (in Russian). 1999; (2-3); 102-106.
36. Limanska O.Yu., Limansky A.P. Embryo sexing and bovine leukemia virus detection by polymerase chain reaction. Agrarian Sciences (in Russian). 2001; (3); 34-39.
37. Limansky A.P., Limanska O.Yu. Molecular genetic methods as fundament of bovine leucosis eradication programmes. Biotechnology. (in Russian). 2001; (3); 40-50.
38. Limansky A.P. Identification of hypervariable and conservative locuses of Listeria sp. genome. Zh. Microbiol. (Moscow): 2001; (5).
39. Limansky A. Cruciform structure in supercoiled DNA: imaging by atomic force microscopy and computer modeling/ 8th International Symposium on Molecular Aspects of Chemotherapy. September 5-9, 2001; (Gdansk); Poland; P.192.
40. Limansky A. Atomic force microscopy aminomodified tips and substrates for DNA immobilization/ 8th International Symposium on Molecular Aspects of Chemotherapy. September 5-9, 2001; (Gdansk); Poland; P.193.
41. Limansky A. Imaging of linear and supercoiled nucleic acids in air and cells in aqueous solution by atomic force microscopy/ Molecular biology and genetics. Conference of young scientists. September 20-22, 2001; (Kiev); Ukraine; P.106.
42. Limanska O., Limansky A. Analysis of primer sets for provirus DNA detection by polymerase chain reaction of bovine leukemia virus and human immunodeficiency virus/ Molecular biology and genetica. Conference of young scientists. September 20-22, 2001; (Kiev); Ukraine; P.150.